Walking pattern analysis after unilateral 6-OHDA lesion and transplantation of foetal dopaminergic progenitor cells in rats
Identifieur interne : 002054 ( Main/Exploration ); précédent : 002053; suivant : 002055Walking pattern analysis after unilateral 6-OHDA lesion and transplantation of foetal dopaminergic progenitor cells in rats
Auteurs : Alexander Klein [Canada, Allemagne] ; Johanna Wessolleck [Allemagne] ; Anna Papazoglou [Allemagne] ; Gerlinde A. Metz [Canada] ; Guido Nikkhah [Allemagne]Source :
- Behavioural brain research [ 0166-4328 ] ; 2009.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Functional sensorimotor recovery after transplantation of mesencephalic dopaminergic (DAergic) neurons has been well documented in the rat 6-hydroxydopamine (6-OHDA) model of Parkinson's disease. However, the functional restoration of more specific gait-related patterns such as skilled walking, balance, and individual limb movements have been insufficiently studied. The purpose of this study was to investigate the behavioural effects of intrastriatal DA grafts on different aspects of normal and skilled walking in rats following unilateral 6-OHDA lesions of the medial forebrain bundle. Rats were subjected to drug-induced rotation, detailed footprint analysis, and assessment of skilled walking in the ladder rung walking test prior and after the transplantation of E14 ventral mesencephalon-derived progenitor cells. Good DAergic graft survival, as revealed by immunohistochemistry, was accompanied by a compensation of drug-induced rotational asymmetries. Interestingly, the analysis of walking patterns displayed a heterogeneous graft-induced response in skilled and non-skilled limb use. Grafted animals made fewer errors with their contralateral limbs in skilled walking than the sham-transplanted rats, and they improved their ipsi- and contralateral limb rotation. However, the parameter distance between feet showed a delayed recovery, and the stride length was not affected by the DA grafts at all. These findings indicate that ectopic intrastriatal transplantation of E14 ventral mesencephalon-derived cells promotes recovery of gait balance and stability, but does not ameliorate the shuffling gait pattern associated with 6-OHDA lesions. A full restoration of locomotor gait pattern might require a more complete and organotypic reconstruction of the mesotelencephalic DAergic pathway.
Affiliations:
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Metz</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Canadian Centre for Behavioural Neuroscience, University of Lethbridge, 4401 University Drive</s1><s2>Lethbridge, Alberta T1K 3M4</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Lethbridge, Alberta T1K 3M4</wicri:noRegion></affiliation></author><author><name sortKey="Nikkhah, Guido" sort="Nikkhah, Guido" uniqKey="Nikkhah G" first="Guido" last="Nikkhah">Guido Nikkhah</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratory of Molecular Neurosurgery, Department of Stereotactic Neurosurgery, University Hospital -Neurocentre, Breisacher Str. 64</s1><s2>79106 Freiburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Behavioural brain research</title><title level="j" type="abbreviated">Behav. brain res.</title><idno type="ISSN">0166-4328</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Behavioural brain research</title><title level="j" type="abbreviated">Behav. brain res.</title><idno type="ISSN">0166-4328</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term><term>Dopamine</term><term>Gait</term><term>Graft</term><term>Lesion</term><term>Midbrain</term><term>Oxidopamine</term><term>Parkinson disease</term><term>Precursor cell</term><term>Progenitor cell</term><term>Rat</term><term>Transplantation</term><term>Walking</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Marche à pied</term><term>Oxidopamine</term><term>Lésion</term><term>Transplantation</term><term>Dopamine</term><term>Cellule précurseur</term><term>Cellule progéniteur</term><term>Maladie de Parkinson</term><term>Allure</term><term>Mésencéphale</term><term>Greffe</term><term>Rat</term><term>Animal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Functional sensorimotor recovery after transplantation of mesencephalic dopaminergic (DAergic) neurons has been well documented in the rat 6-hydroxydopamine (6-OHDA) model of Parkinson's disease. However, the functional restoration of more specific gait-related patterns such as skilled walking, balance, and individual limb movements have been insufficiently studied. The purpose of this study was to investigate the behavioural effects of intrastriatal DA grafts on different aspects of normal and skilled walking in rats following unilateral 6-OHDA lesions of the medial forebrain bundle. Rats were subjected to drug-induced rotation, detailed footprint analysis, and assessment of skilled walking in the ladder rung walking test prior and after the transplantation of E14 ventral mesencephalon-derived progenitor cells. Good DAergic graft survival, as revealed by immunohistochemistry, was accompanied by a compensation of drug-induced rotational asymmetries. Interestingly, the analysis of walking patterns displayed a heterogeneous graft-induced response in skilled and non-skilled limb use. Grafted animals made fewer errors with their contralateral limbs in skilled walking than the sham-transplanted rats, and they improved their ipsi- and contralateral limb rotation. However, the parameter distance between feet showed a delayed recovery, and the stride length was not affected by the DA grafts at all. These findings indicate that ectopic intrastriatal transplantation of E14 ventral mesencephalon-derived cells promotes recovery of gait balance and stability, but does not ameliorate the shuffling gait pattern associated with 6-OHDA lesions. A full restoration of locomotor gait pattern might require a more complete and organotypic reconstruction of the mesotelencephalic DAergic pathway.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Canada</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><country name="Canada"><noRegion><name sortKey="Klein, Alexander" sort="Klein, Alexander" uniqKey="Klein A" first="Alexander" last="Klein">Alexander Klein</name></noRegion><name sortKey="Metz, Gerlinde A" sort="Metz, Gerlinde A" uniqKey="Metz G" first="Gerlinde A." last="Metz">Gerlinde A. Metz</name></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Klein, Alexander" sort="Klein, Alexander" uniqKey="Klein A" first="Alexander" last="Klein">Alexander Klein</name></region><name sortKey="Nikkhah, Guido" sort="Nikkhah, Guido" uniqKey="Nikkhah G" first="Guido" last="Nikkhah">Guido Nikkhah</name><name sortKey="Papazoglou, Anna" sort="Papazoglou, Anna" uniqKey="Papazoglou A" first="Anna" last="Papazoglou">Anna Papazoglou</name><name sortKey="Wessolleck, Johanna" sort="Wessolleck, Johanna" uniqKey="Wessolleck J" first="Johanna" last="Wessolleck">Johanna Wessolleck</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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